alias	title	scientific_name	sample_description	sample_attribute[age]	cell_type	dev_stage	sample_attribute[genetic modification]	sample_attribute[genotype]	sample_attribute[initial time point]	sample_attribute[organism part]	sex	strain	collection date	geographic location (country and/or sea)
7c8760a8-1848-4d45-b963-a933d64753db	49_KO_Rep1	Mus musculus	Mouse brains were dissected and processed to obtain primary neuronal cells. Tissue was enzymatically digested and mechanically dissociated into a single-cell suspension. Cells were filtered, counted, and prepared for downstream culture. ; Primary neurons were cultured for two weeks under controlled conditions. Cells were maintained in specialized medium with regular partial medium changes. Culture conditions supported neuronal maturation and network formation. A targeted knockout of Brpf1 was introduced using CRISPR-Cas9 (KO samples). Neuronal cultures were transfected with sgRNA constructs targeting the gene. Edited cells were maintained for downstream transcriptomic analysis.	20 weeks	Neuron	adult	gene knock out	Brpf1-	birth	brain	female	BALB/c	not provided	not applicable
ef3e12e6-8c5a-48a0-a956-7da24d4ddfc3	49_KO_Rep2	Mus musculus	Mouse brains were dissected and processed to obtain primary neuronal cells. Tissue was enzymatically digested and mechanically dissociated into a single-cell suspension. Cells were filtered, counted, and prepared for downstream culture. ; Primary neurons were cultured for two weeks under controlled conditions. Cells were maintained in specialized medium with regular partial medium changes. Culture conditions supported neuronal maturation and network formation. A targeted knockout of Brpf1 was introduced using CRISPR-Cas9 (KO samples). Neuronal cultures were transfected with sgRNA constructs targeting the gene. Edited cells were maintained for downstream transcriptomic analysis.	20 weeks	Neuron	adult	gene knock out	Brpf1-	birth	brain	female	BALB/c	not provided	not applicable
b1513eb5-f3d3-4e5d-9bd3-400e9c4d50de	49_WT_Rep1	Mus musculus	Mouse brains were dissected and processed to obtain primary neuronal cells. Tissue was enzymatically digested and mechanically dissociated into a single-cell suspension. Cells were filtered, counted, and prepared for downstream culture. ; Primary neurons were cultured for two weeks under controlled conditions. Cells were maintained in specialized medium with regular partial medium changes. Culture conditions supported neuronal maturation and network formation. A targeted knockout of Brpf1 was introduced using CRISPR-Cas9 (KO samples). Neuronal cultures were transfected with sgRNA constructs targeting the gene. Edited cells were maintained for downstream transcriptomic analysis.	20 weeks	Neuron	adult	none	wild type	birth	brain	female	BALB/c	not provided	not applicable
19e0df52-c2bd-40bc-a982-811a7f076a10	49_WT_Rep2	Mus musculus	Mouse brains were dissected and processed to obtain primary neuronal cells. Tissue was enzymatically digested and mechanically dissociated into a single-cell suspension. Cells were filtered, counted, and prepared for downstream culture. ; Primary neurons were cultured for two weeks under controlled conditions. Cells were maintained in specialized medium with regular partial medium changes. Culture conditions supported neuronal maturation and network formation. A targeted knockout of Brpf1 was introduced using CRISPR-Cas9 (KO samples). Neuronal cultures were transfected with sgRNA constructs targeting the gene. Edited cells were maintained for downstream transcriptomic analysis.	20 weeks	Neuron	adult	none	wild type	birth	brain	female	BALB/c	not provided	not applicable